Biochem/physiol Actions

Kd = 15 nM for actin binding

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetF-actin

General description

A cell-permeable F-actin probe. This macrocyclic cyclodepsipeptide, isolated from the marine sponge Jaspis johnstoni, is a potent inducer of actin polymerization and stabilization in vitro. It competes with Phalloidin (Cat. No. 516640) for actin binding (K_d = 15 nM) and enhances apoptosis induced by interleukin 2 (IL-2) deprivation. Also displays fungicidal, insecticidal, and anticancer properties.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Rosado, J.A., et al. 2000. J. Biol. Chem.275, 7527.Posey, S.C., and Bierer, B.E. 1999. J. Biol. Chem.274, 4259.Senderowicz, A.M., et al. 1995. J. Natl. Cancer Inst.87, 46.Bubb, M.R., et al. 1994. J. Biol. Chem.269, 14869.Scott, V.R., et al. 1988. Antimicrob. Agents Chemother.32, 1154.

Packaging

Packaged under inert gas

50 µg in Plastic ampoule

Physical form

A 1 mM (50 µg/71 µl) solution of Jasplakinolide, Jaspis johnstoni (Cat. No. 420107) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Harmful (C)